COVID-19: Clinical Pathways to Pandemic Relief

By Graeme Gardner, Ph.
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Submitted by davidw on Thu Mar 26, 2020 3:03 PM

Space Biology: A Road to the Moon, Mars, and Beyond

It’s an exciting time in the field of space travel research.
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Submitted by on Wed Feb 26, 2020 5:05 PM

Book Review - Bad Blood - Secrets and Lies in a Silicon Valley Startup

Bad Blood: Secrets and Lies in a Silicon Valley Startup by John Carreyrou chronicles the rise and fall of multibillion-dollar biotech startup Theranos.
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Submitted by on Thu Dec 12, 2019 5:05 PM

Harnessing the microbiome potential

Spring is underway in parts of the US, and while Florida by no means undergoes the dramatic reappearance of foliage or colorful clumps of daffodils and azaleas, the southern part of the sunshine state experiences its share of spring renewals.
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Submitted by sheenak on Fri Mar 31, 2017 5:05 PM

ORB relocates to Deerfield Beach and celebrates grand opening of new facility with local researchers

After 8 years of operation in Palm Beach Gardens, Ocean Ridge Biosciences (ORB) has recently relocated to a 5,394 sq.
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Submitted by oceanrid on Mon Nov 21, 2016 2:02 PM

Circulating tumor DNA evaluated as an alternative to tissue NGS for non-small cell lung cancer

Tissue biopsies, which are currently the gold standard molecular diagnostic technique for oncologists, are not only painful to cancer patients, but expose an already immune-comprised population to increased rates of nosocomial infections.
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Submitted by sheenak on Fri Oct 21, 2016 1:01 PM

FierceBiotech's 2016 Fierce 15

It's that time of year again when new school routines are established, deciduous trees begin their showy transformation, and FierceBiotech releases its annual list of 15 innovative companies who are primed to make a big splash in the biopharma industry.
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Submitted by sheenak on Fri Sep 23, 2016 1:01 PM

Happy Birthday Charles

February 12th, 1809, Shrewsbury, England (UK).
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Submitted by on Fri Feb 12, 2016 2:02 PM

Palm Beach State Students tour Ocean Ridge Biosciences (2014-12-08)

On Monday November 25th, 2014 Ocean Ridge Biosciences (ORB) hosted a tour of the lab facility for Palm Beach State Biotechnology students.
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Submitted by sheenak on Mon Dec 8, 2014 1:01 PM

Characterization of small non-coding RNAs in human seminal exosomes. (2014-06-30)

Exosomes, extracellular microvesicles of 30-200 nanometers in diameter, have been detected in a wide variety of biological fluids including serum, saliva, urine and lymph. These microvesicles have been shown to play an important role in intercellular signaling as well as normal cellular physiology (Tsai et. al, 2014). In cancer biology, tumor cells can utilize exosomes to condition a tissue site for metastasis, and to block the cellular immune response by inducing apoptosis in cytotoxic T cells and by reducing the proliferation of NK cells (Tickner et. al, 2014). Exosomes from human semen likely originate from multiple cell types within the male reproductive tract and have been demonstrated to impair immune cell function; this activity may mediate the localized immunosuppressive effects of semen (Vojtech et. al, 2014).

RNA extracted from exosomes has been extensively characterized by second generation sequencing; up to 50% of sequence reads are derived from microRNA, with a wide variety of additional non-coding RNA classes making up the balance (Cheng et. al, 2014). Recently, Lucia Vojtech and co-workers reported the first analysis of RNA content within seminal exosomes. The article entitled: Exosomes in human semen carry a distinctive repertoire of small non-coding RNAs with potential regulatory functions was published in Volume 42, Issue 11 (2014) addition of Nucleic Acids Research. Ocean Ridge Biosciences (ORB) was honored to perform the contract library preparation, sequencing, and initial analysis for the publication.

One of the interesting aspects of the study was the sequencing of two separate size fractions of small RNAs from the seminal exosome RNA preparations: 15-40, and 40-100 nucleotides. ORB scientists enabled this analysis by performing acrylamide gel fractionation of small RNA libraries following the ligation of single-stranded adapters to the target RNA, and again following the production of double-stranded DNA libraries by limited cycles of PCR.  The effectiveness of the size fractionation of the libraries was demonstrated by the markedly different RNA biotype composition of the two fractions demonstrated by the analysis of the sequence reads. Whereas the larger size fraction was comprised predominately of fragments of rRNA and miscellaneous coding and non-coding long RNAs; the smaller fraction contained a higher representation of mature microRNA, Y RNA, and tRNA fragments.

Through additional bioinformatics analysis of the sequence reads, Vojtech and co-workers were able to identify several types of RNAs in human seminal exosomes which may impact target immune system cells by modulating protein translation. These include specific microRNAs, the 5’-cleavage products of tRNA and Y RNA, and small fragments from the untranslated region of protein-coding mRNAs. Together, the results give rise to a testable hypothesis that specific small RNAs in seminal exosomes may mediate immunosuppressive activity of semen by regulating translation of specific genes in the immune system cells of the female reproductive partner.

Submitted by davidw on Mon Jun 30, 2014 1:01 PM